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. Author manuscript; available in PMC: 2009 Mar 2.
Published in final edited form as: J Cell Biochem. 2005 Aug 1;95(5):1057–1068. doi: 10.1002/jcb.20466

Fig. 1.

Fig. 1

Type X collagen and Wnt gene expression in chick chondrocyte cultures. Chondrocytes were isolated from the cephalic (upper) or caudal (lower) sternum or tibia growth plates of day 15 and day 19 chicken embryos as described in Materials and Methods. Total RNA was prepared from cultures at days 2, 4, and 6. Real-time RT-PCR was carried out using primers for chicken type X collagen, Wnt4, Wnt5a, Wnt7a, Wnt8c, and Wnt9a. The relative expression of colX was examined over time in the different cultures (A). Wnt expression was examined over time in lower sternal chondrocytes (B), upper sternal chondrocytes (C), and growth plate chondrocytes (D). Gene expression was normalized to GAPDH. The data shown are representative results of three independent experiments; bars, ±standard error of the mean.