Transforming growth factor-beta (TGF-β), parathyroid hormone related peptide (PTHrP), and BMP-2 regulate Wnt expression in Chondrocytes. Embryonic chick cephalic sternal chondrocyte were cultured for 24 h and were treated with either TGF-β or PTHrP or with BMP-2. After 24 h of treatment, total RNA was extracted from the cultures and gene expressions determined by real-time RT-PCR using chick specific primers. The data shown are representative results of three independent experiments; bars, ±standard error of the mean. The symbol, * represents a significant difference compared to controls with P <0.05.