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. Author manuscript; available in PMC: 2009 Mar 2.
Published in final edited form as: J Cell Biochem. 2005 Aug 1;95(5):1057–1068. doi: 10.1002/jcb.20466

Fig. 5.

Fig. 5

BMP-2 enhances β-Catenin-TCF-4 mediated transcription. Embryonic chick upper sternal chondrocyte were transfected with the full-length type X collagen reporter (ABC-640-Luc; 100 ng) and TCF-4 (500 ng) and/or either mutant (non-degradable) or wild-type β-Catenin (500 ng) after 12 h in culture. Two hours after transfection, standard medium was added with or without BMP-2 (50 ng/ml) (A) or TGF-β (5 ng/ml) (B) and the cultures were harvested 48 h later for luciferase assay. A basic PGL3 vector (10 ng) containing the firefly luciferase gene containing the firefly luciferase gene was used to standardize transfection efficiency. Results are represented as relative luciferase activities obtained by dividing the firefly luciferase by renilla luciferase.