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. Author manuscript; available in PMC: 2009 Mar 2.
Published in final edited form as: J Cell Biochem. 2005 Aug 1;95(5):1057–1068. doi: 10.1002/jcb.20466

Fig. 6.

Fig. 6

TGF-β antagonizes β-Catenin-TCF-4 mediated transcription. Similarly, chondrocytes were transfected with the Topflash reporter (500 ng) and β-Catenin (500 ng) alone or with TCF-4 (500 ng) in the presence of TGF-β (5 ng/ml) and luciferase assay performed after 48 h. Transfection efficiency was determined by co-transfection with pRL vector (Promega) and determining the renilla uniformis luciferase activity (A). Embryonic chick upper sternal chondrocyte were transfected with p3TP-Luc (500 ng) and TCF-4 (500 ng) and either mutant wild-type β-Catenin (500 ng) or control vector after 12 h in culture. Two hours after transfection, standard medium was added with or without TGF-β (5 ng/ml) and the cultures were harvested 48 h later for luciferase assay. Transfection efficiency was determined by co-transfection with pRL vector (Promega) and determining the renilla uniformis luciferase activity. Results are represented as relative luciferase activities obtained by dividing the firefly luciferase by renilla luciferase (B).