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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: FEMS Yeast Res. 2008 Dec;8(8):1223–1235. doi: 10.1111/j.1567-1364.2008.00441.x

FIG. 3.

FIG. 3

(Panels A and B) Influence of rapmycin treatment and nitrogen source on the intracellular distribution of Gat1-GFP and Gln3-GFP. The nitrogen sources provided were glutamine (Gln) or proline (Pro) as indicated. Two of the cultures were treated with rapamycin (Gln+Rap) as described in Materials and Methods. (Panels C and D) NaCl elicits relocalization of Gat1-GFP and Gln3-GFP. The nitrogen sources provided were ammonia (Am.) or proline (Pro). NaCl was added to a final concentration of 1 M for 20 min prior to cell harvest where indicated (Pro + NaCl). The strain used in all four panels was JK9-3da (TB123 background).