Skip to main content
. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: FEMS Yeast Res. 2008 Dec;8(8):1223–1235. doi: 10.1111/j.1567-1364.2008.00441.x

FIG. 4.

FIG. 4

(Panels A and B)The methanol component of commercial formalin preparations has no demonstrable effect on intracellular Gat1-Myc13 localization. The final concentrations (percentage) of formalin (Form.) and methanol (MeOH) used in the fixation procedure are as indicated. Varying methanol concentrations were achieved by adding additional methanol to the formalin solution. The nitrogen source and presence or absence of rapamycin treatment are as indicated. Images are as indicated in Fig. 1. (Panels C and D) The concentration of Para-formaldehyde present during fixation influences intracellular Gat1-Myc13 localization. Para-formaldehyde is indicated as P-form. in histograms and as Para in images. See Materials and Methods for para-formaldehyde preparation. The strain used in all four panels was FV063.