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Design of tandem FRET substrates for BoNT/A-LC assays. Shown is an illustration depicting BoNT/A-mediated cleavage of tandem FRET substrates. Spheres represent CFP (cyan) or YFP (yellow) connected to SNAP substrate peptides (curved lines). Dotted lines indicate intramolecular FRET that is lost upon enzymatic cleavage.
