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. Author manuscript; available in PMC: 2010 Mar 15.
Published in final edited form as: Toxicon. 2009 Jan 13;53(4):392–399. doi: 10.1016/j.toxicon.2008.12.016

Figure 2.

Figure 2

Emission scans of three FRET substrates, CsY, CsYY and YsCsY. Spectra before (solid red lines) and after (dotted blue lines) treatment with BoNT/A-LC were recorded at 30°C using excitation at 420 nm with (A) 10 μM Zn2+ and increasing substrate concentrations of 1, 3 or 5 μM, or (B) 300 μM Zn2+ and increasing substrate concentrations of 2.5, 5 and 10 μM.