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. Author manuscript; available in PMC: 2010 Mar 15.
Published in final edited form as: Toxicon. 2009 Jan 13;53(4):392–399. doi: 10.1016/j.toxicon.2008.12.016

Figure 6.

Figure 6

Continuous monitoring of BoNT/A-LC-catalyzed cleavage of YsCsY. BoNT/A-LC was 2-fold serially diluted at concentrations from 2 nM to 2 pM in reaction buffer containing 10 μM of Zn2+ and incubated in a fluorescence microplate reader at 30°C for 30 min before addition of a solution of 3 μM of YsCsY. The data were recorded in filter mode with excitation at 400/30 nm and emission at 528/20 nm.