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Temperature dependence of BoNT/A-LC catalyzed cleavage of YsCsY. The initial rates were determined from ΔRFU recorded in filter mode (528/20 nm) for reactions with 5 μM of YsCsY, 10 μM of Zn2+ and 1, 2, 5, or 10 nM of BoNT/A-LC at 30°C (squares) or 37°C (circles). In comparison, the initial rates were determined from ΔRFU recorded in filter mode (420/50 nm) for reactions with 5 μM of SNAPtide™ (oAbz/Dnp), 300 μM of Zn2+, and 1, 2, 5, or 10 nM of BoNT/A-LC at 37°C (triangles).
