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. 2000 Feb 4;97(4):1689–1694. doi: 10.1073/pnas.030390097

Figure 1.

Figure 1

Ag-specific proliferation of T cells from MBPGP68–85 (A and C) and MBPRAT68–85 (B and D) primed LEW rats that had received different DNA constructs 4 weeks before immunization i.m. as indicated in Materials and Methods. Each value represents mean values (±SEM) from four LEW rats. Splenocytes were obtained on day 12 postimmunization, and MNC were cultured for 72 h in triplicates in the presence of either MBPGP68–85 (A and B) or MBPRAT68–85 (C and D) at various antigen concentrations or medium alone. Stimulation with MBP89–101 or MOG37–54 did not result in cpm values over background (data not shown). T cell proliferation was assessed by [3H]thymidine incorporation during the last 12 h of culture.