Figure 4.
Distribution of sulfated GAGs with respect to the local collagen fibril orientation. Left Pane – GAG distribution in the AP plane. GAGs in control images had a median angle of 39.88°. After treatment with ChB sulfated GAGs were skewed toward coaxial distributions with a median angle of 29.85°. Middle Pane – GAG distribution in the ML plane. Control and treated cases were similar to those in the AP plane with median sulfated GAG angles in control and treated of 40.25° and 34.01°, respectively. Right Pane – GAG distribution in the plane transverse to the collagen fibrils. Both the control and ChB treated distributions were nearly flat with median GAG angles of 38.67° and 40.96°, respectively. GAG reduction between control and ChB treated cases was significant in each plane (all p ≈ 0.000) and ranged from 80.6–90.3%.