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. Author manuscript; available in PMC: 2009 Nov 11.
Published in final edited form as: Biochemistry. 2008 Oct 18;47(45):11894–11899. doi: 10.1021/bi801211t

Figure 1.

Figure 1

Estimation of Keq by varying S/P and measuring the change in radiolabeled reactant. The reaction was equilibrated using [(14C)1-naphthol]0 = 20 ∝M, and [PAPS]0 = 10 ∝M, and then adding S/P (cold) in different ratios to the reaction mixture. The change in product concentration was monitored using scintillation counting.