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. 2008 Dec 9;328(3):715–722. doi: 10.1124/jpet.108.147330

Fig. 5.

Fig. 5.

Disorazole C1 causes irreversible growth inhibition. A549 cells were treated for 1 h with 10 nM disorazole C1, 1 μM nocodazolem, or DMSO vehicle followed by either washout of compound (wash) or continuous exposure to compound (contin) for 1, 24, 48, 72, and 144 h. Live cells were counted by trypan blue exclusion. The number of viable cells (trypan blue negative) is graphed as the percentage of viable cells per milliliter normalized to the DMSO control. Data are representative of two independent experiments. B, A549 cells were incubated with doxorubicin (50 nM) or disorazole C1 (2 or 10 nM) for 24 (open bars) or 48 (black bars) h, and apoptosis was determined with a TUNEL assay. n = 3; bars, S.E.M.