Figure 6.
Activation of the PKC pathway by PMA pretreatment reversed the inhibitory effect of S1P. T103p53i cells were untreated or pretreated with PMA (0.1 μM) for 1 hr, Gö6976 (1 μM) for 30 min, or Gö6976 (1 μM) for 30 min followed by PMA (0.1 μM) for 1 hr. Cell migration in response to LPA or/and S1P was then tested. Student's t test, P < 0.05; **, P < 0.01; ***, P < 0.001; compared with the correspondent control (Ctrl, the first bar in the same group). Student's t test, +, P < 0.05; ++, P < 0.01; +++, P < 0.001; compared with the correspondent PMA results (0.1 μM PMA, the second bar in the same group).