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. Author manuscript; available in PMC: 2010 Jan 9.
Published in final edited form as: Brain Res. 2008 Nov 1;1247:196–211. doi: 10.1016/j.brainres.2008.10.015

Figure 7. TAM mediated neuronal Vm maintenance is partially mediated by activation of BK channels.

Figure 7

A. Voltage-clamp recordings of CA1 pyramidal neurons exposed to a 15 min.OGD treatment at −60 mV holding potential. As indicated in A, the black, green and orange traces are representative recordings under conditions of OGD, OGD+TAM (10μM) + Pax (10μM) and OGD +TAM (10μM), respectively. The relative speed and degree of Vm depolarization under these recording conditions were proportional to the slope and amplitude of the holding inward currents. The OGD induced large inward current (black) was substantially inhibited by TAM (orange), which was partially inhibited by Pax (green). B. Summary of all the experiments including additional controls of Pax alone in control aCSF and Pax + OGD. Pax alone did not result in a significant negative shift in holding currents (−9.6 ± 5.9 pA). OGD alone induced a −82±8.7 pA negative shift in holding currents that was markedly reduced by TAM (−23 ± 5.7 pA). The TAM effect was partially blocked by Pax (−64.4 ± 6.8 pA). Pax itself markedly negatively shifted OGD induced inward holding currents (−114 ± 16.5 pA). Tukey’s HSD test was performed for multiple comparisons amongst OGD, OGD+TAM, OGD+TAM+Pax and OGD+Pax groups. The significant (p < 0.05) differences between OGD, OGD+TAM and OGD+TAM+Pax with other groups are indicated by **, † and, ‡, respectively. In the presence of Pax, TAM inhibition of OGD induced sEPSCs was largely reversed. C The cumulative fraction of sEPSCs in C was calculated and compared as for Fig. 6. D. the OGD induced sEPSCs increase was only partially inhibited in OGD+TAM+Pax group, 172 ± 26.5/min, as compared to OGD +TAM at 108 ± 28.8/min, all at the end of 15 min. OGD treatment. * in D indicates the difference between OGD and OGD+TAM, or OGD and OGD+TAM+Pax at a given time point during a 15 min. OGD treatment is statistically significant at p < 0.05, determined by one-way ANOVA test.