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Retrosplenial areas are well defined with NeuN, SMI32 and parvalbumin. Indeed, the massive intrinsic parvalbumin system is very robust in area 29l. Neurons in the granular layer (i.e., layer III/IV) do not express NFP and this is documented by merging images from NeuN and SMI32. Area 30 is dysgranular as seen with NeuN and the highest level of NFP expression is in layers IIIc and Va as emphasized in the merged sections. Scale bar, 500 μm.
