. Author manuscript; available in PMC: 2009 Nov 5.

Published in final edited form as: J Am Chem Soc. 2008 Oct 10;130(44):14625–14633. doi: 10.1021/ja803960x

Table 5.

The diagonal interaction energies as determined by Double Mutant Cycles

X	Y	ΔΔG (kcal mol^-1)^a
Trp^b	Ac₄Glc	-0.8
Trp	Ac₄Gal	-0.7
Trp	Ac₃GlcNAc	-0.6^c
Trp	GlcNAc	-0.5^c
Trp	Me₄Glc	-0.8^c

The error in the diagonal interaction energy is ±0.1 kcal mol^-1

Previously reported in Ref 19.

Although the interaction enery for this mutant is similar to that of the Trp-Ac4Glc interaction, the NMR data suggests that an interaction other than the carbohydrate-π interaction is contributing. See text for details.