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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Comp Biochem Physiol B Biochem Mol Biol. 2008 Oct 15;152(2):110–117. doi: 10.1016/j.cbpb.2008.10.004

Figure 6.

Figure 6

Gel filtration in a Sephacryl S-400 column showing the effect of metal chelation before the glue sets on aggregation of proteins from A. subfuscus glue. The sample was collected into 3 mM deferoxamine and 10 mM EDTA in 20 mM Tris (pH 8). The elution profile is shown above the silver stained SDS-PAGE analysis of the fractions. The lanes are marked by the elution volumes of the fractions they contain. The positions of the 15, 40 and 100 kDa proteins are indicated to the left.