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. Author manuscript; available in PMC: 2010 Jan 15.
Published in final edited form as: Neuron. 2009 Jan 15;61(1):101–112. doi: 10.1016/j.neuron.2008.11.009

Figure 5. Blocking the dissociation of stargazin from AMPA receptors prevents concentration-dependent modulation of AMPA receptors.

Figure 5

(A) The topology of GluR1, stargazin and the tandem proteins of GluR1 and stargazin, in which the C-terminus of GluR1 is fused to the N-terminus of stargazin. (B) Stargazin enhanced currents evoked by 5 μM glutamate through GluR1o channels. Oocytes injected with 0.05 ng of the cRNA encoding the GluR1o-stargazin tandem construct responded better than those with GluR1o cRNA (20 ng) alone. Co-expression of stargazin failed to cause a further enhancement of current amplitude. (C) The GluR1o-stargazin tandem protein modulates kainate efficacy to a similar extent as GluR1o and stargazin. The co-expression of STG with the GluR1o-stargazin tandem protein did not produce any additional enhancement of the response. (D) In contrast to receptors expressed in response to the co-expression of GluR1o and stargazin, the GluR1o-stargazin tandem protein did not show a reduction in current amplitudes at high glutamate concentrations. Co-expression of stargazin had no effect on the concentration-response behavior of GluR1o-stargazin receptors. Data represent the mean ± s.e.m. (n=5, 6).