Figure 6.

Stat activation induced by cmvIL-10 in hamster cells and PBMCs. EMSAs were used to measure activation of Stat1 and Stat3. Hamster cells expressing both receptor chains (Fig. 4) and PBMCs were used. Cells were left untreated or treated with recombinant IL-10 (100 units/ml) or with conditioned media (200 μl) from COS cells transfected with the pEF-SPFL-cmvIL-10 plasmid or from uninfected or CMV-infected cells. Cellular lysates were prepared and assayed for Stat activation in the EMSA as described in Materials and Methods. Positions of Stat DNA-binding complexes are indicated by arrows. Antibodies against Stat1 and Stat3 were added as indicated to reduce the mobility of complexes containing these proteins.