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An example of validation of intercross-link determination and peptide sequence identification for the precursor ion with neutral mass of 4884.3531. a, isolation and MS2 of precursor ion (m/z 9785+). All peaks produced from cleavage of one and two PIR labile bonds are labeled schematically. b, isolation and MS3 of the released peptide ion with m/z 12432+ and 1280+, respectively.
