Fig. 2.

Fold-change distributions of the proteome. A, quantitative comparison of the primary against the Hepa1–6 cell line proteome. The distribution was divided into five quantiles as follows. High relative expression in primary cells (0–15%, at least 4-fold down-regulation), mostly expressed in primary cells (15–25%, −4 to −1.5-fold regulation), not highly regulated proteins (25–75%; −1.5 to +2.8), mostly expressed in Hepa1–6 (75–85; 2.8 to 3.6-fold), highly expressed in Hepa1–6 (85–100%, more than 3.6-fold change). Color coding of these categories is indicated at the top of the panel. B, biological replicate of the experiment showing excellent reproducibility (see also supplemental Fig. 2). C, quantitative comparison of the Hepa1–6 proteome cultured in high glucose (28 nm) and physiological glucose concentration (8 nm).