Fig. 4.

Phenotypic proteome comparison at the cellular component level. A, the quantiles resulting from quantitative proteome comparison in Fig. 2 were separately analyzed for enriched gene ontology cellular components and clustered for the z-transformed p values. The color bar on top represents the quantiles. Representative categories enriched in the protein population of each quantile are annotated. For complete listing of significant categories and p values, see supplemental Fig. 3. Prominent mitochondria-related categories for the primary cells are highlighted in red and prominent nucleus related categories in blue. B, nuclear (DAPI) and mitochondrial (Mitotracker) staining of primary hepatocytes and Hepa1–6 cells. Most primary hepatocytes are binuclear (34). Magnification factor is ×400. DAPI, 4′,6-diamidino-2-phenylindole.