Fig. 2.

Enhanced expression of proteins involved in information storage and processing pathways in wild type D. radiodurans in response to γ radiation (1 kGy). R1, wild type strain without irradiation; YR1, pprI knock-out strain without irradiation; R1 γ+, wild type strain with irradiation; YR1 γ+, pprI knock-out strain with irradiation. Protein spots in 2-D gels are shown for DR0099, DR2340, and DRA0346 (SsB, RecA, and PprA, respectively) (A); DR0307 and DR1082 (elongation factor G and light-repressed protein A, respectively) (B); and DR1473 and DR2128 (phage shock protein A and DNA-directed RNA polymerase α subunit, respectively) (C). D, relative protein expression levels (see “Experimental Procedures” for calculations). Each data point represents an average of quadruplicate experiments, and error bars represent standard deviations.