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. 2009 Mar;8(3):481–494. doi: 10.1074/mcp.M800123-MCP200

Fig. 4.

Fig. 4.

Enhanced expression of proteins cataloged in the D. radiodurans metabolic pathways in response to γ radiation (1 kGy). R1, wild type strain without irradiation; YR1, pprI knock-out strain without irradiation; R1 γ+, wild type strain with irradiation; YR1 γ+, pprI knock-out strain with irradiation. A, protein spots in 2-D gels for DR0435 (cytochrome complex iron-sulfur), DR0697 (V-type ATP synthase, E subunit), DR0700 (V-type ATP synthase, A subunit), DR2075 (ferredoxin), DR2317 (potassium channel β subunit), and DR2370 (pyruvate dehydrogenase complex). The six proteins are cataloged in the subgroup of energy production and conversion. B, DR1519 (ketol-acid reductoisomerase) in 2-D gels. C, DR2117 and DRA0018 (adenylate kinase and 5`-nucleotidase, respectively). D, DR0114 and DR2361 (enoyl-CoA hydratase and acyl-CoA dehydrogenase, respectively). E, DR1127 and DR1149 (hypothetical protein and Na+/H+ antiporter, respectively). F, relative expression levels of proteins (see “Experimental Procedures” for calculations). Each data point represents an average of quadruplicate experiments, and error bars represent standard deviations.