Fig. 5.

Enhanced expression of proteins involved in the poorly characterized group in wild type D. radiodurans in response to γ radiation (1 kGy). R1, wild type strain without irradiation; YR1, pprI knock-out strain without irradiation; R1 γ+, wild type strain with irradiation; YR1 γ+, pprI knock-out strain with irradiation. A, protein spots in 2-D gels for DR1598 and DR2557 (protease and hypothetical protein, respectively). B, spots of four hypothetical proteins in 2-D gels, DR0459, DR0581, DR1448, and DR2373, that are not cataloged by COGs. C, relative expression levels of proteins (see “Experimental Procedures” for calculations). Each data point represents an average of quadruplicate experiments, and error bars represent standard deviations.