Fig. 6.

Remarkable reduction in protein expression in D. radiodurans in response to γ radiation (1 kGy). R1, wild type strain without irradiation; YR1, pprI knock-out strain without irradiation; R1 γ+, wild type strain with irradiation; YR1 γ+, pprI knock-out strain with irradiation. A, protein spots in 2-D gels for DR1983 (ribosomal protein S1), DR0757 (citrate synthase), DRA0201 (NH₃-dependent NAD⁺ synthase), DR0969 (hypothetical protein), and DR2337 (hypothetical protein). B, relative expression levels of proteins (see “Experimental Procedures” for calculations). Each data point represents an average of quadruplicate experiments and error bars represent standard deviations.