Skip to main content
. 2009 Mar;8(3):535–546. doi: 10.1074/mcp.M800340-MCP200

Fig. 5.

Fig. 5.

FIH-dependent hydroxylation at Asn-427 in Tankyrase-2. Extracted ion chromatograms of m/z 513.27 and m/z 518.60 corresponding to unmodified and hydroxylated forms of the tryptic Tankyrase-2 peptide VNALDNLGQTSLHR ([M + 3H]3+). Nano-UPLC-MSE chromatography analysis illustrating the relative abundance of hydroxylation at Asn-427 following FIH intervention: FIH RNA interference knockdown (lower panel; <1%), endogenous levels of FIH (middle panel; ∼7%), and FIH overexpression (upper panel; ∼70%) is shown.