Table I.

Identification of FIH-interacting ARD protein candidates using a SILAC-based proteomics screen

Protein	Accession no. (Swiss-Prot)	Protein scorea	MS/MS mode	Sequence coverage	Peptides total	Heavyb	Lightc
				%
Factor inhibiting HIFd	Q9NWT6	3652	DDA	77	129	63	66
Rabankyrin-5e	Q9P2R3	491	DDA	12	9	9	0
		138	MSE	25	18	18	2
Ankyrin repeat and KH domain-1	Q8IWZ3	90	DDA	2	6	5	1
Tankyrase-2e	Q9H2K2	51	DDA	2	3	3	0
		41	MSE	10	12	9	3
Ankyrin repeat domain-52	Q8NB46	37	MSE	11	11	6	5
Notch2f	Q04721	22	DDA	1	1	1	0
Ankyrin repeat domain-27	Q96NW4	21	MSE	7	6	4	2
Ankyrin repeat and SAM domain-1	Q92625	21	MSE	4	3	2	1
Ankyrin repeat domain-60g	Q9BZ19	16	MSE	30	12	10	2
Ankyrin repeat domain-35g	Q8N283	13	MSE	19	23	15	8
IκBεg	Q14164	11	MSE	8	6	5	1

^aMascot (DDA), PLGS (MS^E).

^bDerived from sample treated with DMOG.

^cDerived from untreated sample.

^dBait protein used as internal standard for an equal ratio of heavy to light peptides.

^eFIH interaction of this protein confirmed in this study.

^fShown previously to be on FIH substrate (4).

^gIncluding peptide/protein probabilities of <50% as calculated by PLGS.