Table II.
Antioxidant activities of peptides (80 μg/ml)
Values are means ± S.D. of three replicate analyses. NA, no activity.
| Samples | Free radical scavenging capacity
|
Fe3+ reducing power (absorbance at 700 nm) | ||
|---|---|---|---|---|
| ABTS+ | DPPH | NO | ||
| % | ||||
| H2O | 0 ± 0.0 | 0 ± 6.22 | NA | NA |
| BHA | 70.1 ± 6.5 | 90.4 ± 13.9 | 46.5 ± 7.4 | 0.27 ± 0.06 |
| NFAT inhibitor | 15.2 ± 1.7 | 9.5 ± 5.4 | NA | 0.06 ± 0.02 |
| Galanin (porcine) fragment 1–16 | 8.9 ± 3.3 | 8.2 ± 5.5 | NA | 0.06 ± 0.01 |
| Somatostatin fragment 9–23 | 17.6 ± 3.6 | 11.3 ± 2.2 | 2.9 ± 0.3 | 0.08 ± 0.04 |
| Pleurain-A1 | 74.8 ± 2.2 | NA | 21.1 ± 5.2 | 0.15 ± 0.04 |
| M5G-pleurain-A1 | 49.6 ± 4.3 | NA | 10.7 ± 3.6 | 0.09 ± 0.02 |
| P12G-pleurain-A1 | 60.1 ± 4.8 | NA | 3.1 ± 0.3 | 0.13 ± 0.04 |
| W15G-pleurain-A1 | 70.7 ± 2.0 | NA | 11.3 ± 2.7 | 0.12 ± 0.07 |
| Y23G-pleurain-A1 | 37.1 ± 3.4 | NA | 5.5 ± 1.9 | 0.07 ± 0.01 |
| M5G/P12G/W15G/Y23G-pleurain-A1 | No antioxidant activity | |||
| Pleurain-D4 | NA | 30.3 ± 12.7 | 35.7 ± 3.6 | 0.21 ± 0.02 |
| P13G-pleurain-D4 | NA | 5.1 ± 1.3 | 2.7 ± 0.3 | 0.03 ± 0.01 |
| Pleurain-E1 | 6.3 ± 8.5 | 69.1 ± 9.6 | 11.5 ± 2.9 | 0.29 ± 0.02 |
| W4G-pleurain-E1 | 5.7 ± 2.4 | 52.3 ± 3.2 | 10.7 ± 4.5 | 0.24 ± 0.05 |
| P7G/P8G/P12G/P16G/P24G-pleurain-E1 | 3.3 ± 0.5 | 27.6 ± 5.7 | 5.1 ± 3.2 | 0.11 ± 0.04 |
| C23G-pleurain-E1 | 2.5 ± 0.7 | 20.4 ± 2.9 | 2.9 ± 1.3 | 0.09 ± 0.01 |
| W4G/P7G/P8G/P12G/P16G/P24G/C23G-pleurain-E1 | No antioxidant activity | |||
| Pleurain-G1 | 66.3 ± 1.8 | 58.5 ± 3.3 | 41.3 ± 7.7 | 0.19 ± 0.01 |
| W3G-pleurain-G1 | 41.7 ± 8.2 | 30.7 ± 4.5 | 27.1 ± 2.2 | 0.12 ± 0.03 |
| P29G/P30G-pleurain-G1 | 30.1 ± 3.9 | 21.5 ± 2.6 | 19.2 ± 5.3 | 0.08 ± 0.05 |
| W3G/P29G/P30G-pleurain-G1 | NA | 5.6 ± 1.4 | NA | 0.02 ± 0.01 |
| Pleurain-J1 | 60.1 ± 0.0 | 76.4 ± 9.6 | NA | 0.37 ± 0.04 |
| P3G/P14G/P24G/P25G-pleurain-J1 | 23.6 ± 2.5 | 25.9 ± 4.4 | NA | 0.15 ± 0.03 |
| C18G-pleurain-J1 | 20.2 ± 3.2 | 19.9 ± 6.7 | NA | 0.11 ± 0.05 |
| P3G/P14G/P24G/P25G/C18G-pleurain-J1 | No antioxidant activity | |||
| Pleurain-K1 | 18.6 ± 2.2 | 13.6 ± 3.9 | NA | 0.24 ± 0.11 |
| P3G-pleurain-K1 | 11.3 ± 2.9 | 9.2 ± 1.8 | NA | 0.17 ± 0.06 |
| M7G-pleurain-K1 | 6.5 ± 1.3 | NA | NA | 0.09 ± 0.01 |
| W10G-pleurain-K1 | 15.1 ± 2.6 | 10.9 ± 3.3 | NA | 0.21 ± 0.05 |
| P3G/M7G/W10G-pleurain-K1 | No antioxidant activity | |||
| Pleurain-M1 | 29.8 ± 0.0 | 31.1 ± 17.3 | NA | 0.13 ± 0.00 |
| P30G-pleurain-M1 | 3.1 ± 1.1 | NA | NA | 0.01 ± 0.00 |
| Pleurain-N1 | 87.8 ± 1.5 | 91.4 ± 3.3 | 52.4 ± 11.2 | 0.35 ± 0.04 |
| C23G-pleurain-N1 | 40.3 ± 3.7 | 42.1 ± 2.2 | 30.6 ± 1.0 | 0.16 ± 0.02 |
| P26G/P29G/P30G-pleurain-N1 | 30.5 ± 5.9 | 27.7 ± 4.5 | 25.4 ± 3.8 | 0.13 ± 0.05 |
| C23G/P26G/P29G/P30G-pleurain-N1 | No antioxidant activity | |||
| Pleurain-P1 | 41.9 ± 2.0 | 83.9 ± 3.8 | 33.1 ± 6.2 | 0.23 ± 0.06 |
| Y24G-pleurain-P1 | 17.3 ± 3.5 | 37.6 ± 7.2 | 17.1 ± 2.9 | 0.10 ± 0.02 |
| P27G/P36G-pleurain-P1 | 20.5 ± 6.7 | 45.3 ± 3.2 | 22.2 ± 4.7 | 0.15 ± 0.01 |
| Y24G/P27G/P36G-pleurain-P1 | 10.1 ± 0.5 | 13.8 ± 4.1 | NA | 0.06 ± 0.01 |
| Pleurain-R1 | 92.4 ± 1.5 | 21.4 ± 3.0 | 17.5 ± 3.9 | 0.34 ± 0.09 |
| W4G-pleurain-R1 | 79.3 ± 9.9 | 20.6 ± 5.1 | 15.6 ± 2.1 | 0.31 ± 0.03 |
| M5G-pleurain-R1 | 38.7 ± 6.2 | 13.3 ± 4.9 | 8.2 ± 1.6 | 0.16 ± 0.05 |
| P16G-pleurain-R1 | 45.6 ± 9.5 | 11.3 ± 5.8 | 9.6 ± 3.4 | 0.18 ± 0.01 |
| W4G/M5G/P16G-pleurain-R1 | No antioxidant activity | |||
| Antioxidin-RP1 | 95.9 ± 4.7 | 96.6 ± 5.1 | 66.4 ± 9.8 | 0.41 ± 0.07 |
| M2G-antioxidin-RP1 | 73.5 ± 8.2 | 72.7 ± 10.5 | 50.1 ± 6.7 | 0.36 ± 0.02 |
| Y6G-antioxidin-RP1 | 55.5 ± 2.1 | 46.8 ± 3.5 | 32.4 ± 3.3 | 0.23 ± 0.05 |
| Y12G-antioxidin-RP1 | 85.7 ± 7.1 | 79.6 ± 9.5 | 52.6 ± 6.2 | 0.37 ± 0.07 |
| P9G-antioxidin-RP1 | 82.1 ± 4.9 | 70.5 ± 2.8 | 45.0 ± 2.9 | 0.37 ± 0.01 |
| C10G-antioxidin-RP1 | 50.1 ± 2.2 | 42.3 ± 5.6 | 27.4 ± 5.5 | 0.22 ± 0.00 |
| M2G/Y6G/Y12G/P9G/C10G-antioxidin-RP1 | No antioxidant activity | |||