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Genus-specific PCR. Molecular diagnostic assays based on PCR amplification of different genomic targets for the identification of Brucella spp. tested of the isolate. The clinical isolate was tested by the conventional biochemical tests and detected with common genes (BCSP-31 kDa, OMP2-36kDa, 16S rRNA) of Brucella spp. M, 100 bp marker; Lane 1, Brucella abortus ATCC 2308; Lane 2, Patient's isolate.
