Fig. 5. The disassembly kinetics of FtsZ polymer following the addition of excess GDP.
a, in MMK buffer, the decay constant for disassembly is 6 s. b, in MEK buffer, the decay constant for disassembly is 21 s. c, the disassembly of FtsZ-L68W assayed by the decrease in tryptophan fluorescence. The average decay constant is ∼120 s. FtsZ was at 6 μM.