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A novel direct binding assay. Sera positive or negative for anti-tTG antibody were directly incubated with intact placental villous tissue fragments and followed by FITC-conjugated anti-IgA. Tissue was then cryosectioned and counterstained for nuclei (PI; red). A) Positive control: mouse monoclonal anti tTG; B) serum positive for anti tTG; C) serum negative for anti-tTG. The green staining on the outer surface of the villus corresponds to syncytium. Scale bar, 100 μm.
