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E10 Lys interactions with the distal His and heme propionate atoms in bovine Hb. A) The large positively charged portion of E10 interacts with the heme propionate oxygen atom and the distal His through separate water molecules in bovine Hb at pH 6.3 in the α subunit. B) E10 interacts directly with the distal His in the bovine Hb at pH 8.5 in the β subunit.
