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. Author manuscript; available in PMC: 2010 Mar 15.
Published in final edited form as: Dev Biol. 2008 Dec 11;327(2):280–287. doi: 10.1016/j.ydbio.2008.11.038

Fig. 5. Fyn PTK activity is required for meiotic maturation of mouse oocytes.

Fig. 5

Oocytes injected with FYN siRNA were held arrested at the GV stage in 300 μM cAMP for 4–5 h, washed in cAMP-free media, and cultured in maturation media for 16–17 h. Controls included injection with an equal concentration of scrambled control siRNA or processing in the above solutions without injection. Seven replicates with siRNA were conducted using both types of controls. Oocytes were labeled for microtubules and DNA as described above (fig. 3) and scored to determine meiotic stage. Injection of FYN siRNA into GV stage oocytes significantly decreased meiotic maturation since fewer FYN siRNA oocytes reached metaphase of MI compared to control siRNA injected or non-injected oocytes (83% versus 100% and 100%, respectively; *P<0.05). Maturation to MII was also impaired with only 39% of FYN siRNA injected oocytes reaching metaphase-II as compared to control siRNA and non-injected oocytes (86% and 93%, respectively; **P<0.05).