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. 2009 Jan 14;100(3):434–440. doi: 10.1111/j.1349-7006.2009.01076.x

Figure 1.

Figure 1

Secretory leukocyte protease inhibitor (SLPI) but not protease‐inhibitor null (PI‐) transfectants reduces plasmin activity. (A) HA‐tagged SLPI forms are found in Hey‐A8 transfectant lysates and conditioned medium (CM). (B) Wild type (WT)/SLPI but not mock or PI‐ cell CM reduce plasminogen cleavage (P ≤ 0.0001). (C) No difference between WT/SLPI and PI‐/SLPI cells in progranulin (PRGN) binding. Co‐immunoprecipitation of PRGN with SLPI is shown. An isotype‐matched polyclonal antibody (pAb) (IgG) confirmed specificity of immunoprecipitation (PI). F‐SLPI, PI‐/SLPI mutant Leu72Phe; R‐SLPI, PI‐/SLPI mutant Leu72Arg; PLG, Plasminogen.