Figure 3.

Protection from anoikis for WT/secretory leukocyte protease inhibitor (SLPI) and protease‐inhibitor null (PI‐) mutants. (A) SLPI transfectants grew more successfully when cultured in serum‐free medium on poly‐hydroxyethylmethacrylate (poly‐HEMA)‐treated plastic. XTT assay was used to quantitate spheroid proliferation (n = 2). (B, C) Increased S phase and decreased sub G_oG₁ fraction in SLPI transfectants. The sub‐G₀G₁‐phase is used as a marker of apoptotic cells. Representative data of three experiments are shown. All P‐values are compared to the mock transfection control. F‐SLPI, PI‐/SLPI mutant Leu72Phe; R‐SLPI, PI‐/SLPI mutant Leu72Arg.