Figure 3.

In the absence of Fas-mediated cell death, the accumulation of apoptotic and dead cells is delayed in populations of CD4⁺ but not CD8⁺ T cells. (A) Early apoptotic and late apoptotic/dead cells were distinguished by 7AAD and Annexin-V staining (Right). Markers were set by control staining in the absence of calcium (Left). (B and C) The percent early apoptotic cells (live-gated Annexin-V⁺ 7AAD⁻) is depicted from days 0 to 5 of culture among B6- and B6.lpr-derived anti-CD3 stimulated splenic CD8⁺ and CD4⁺ T cells, respectively. (D and E) The percent late apoptotic/dead cells (ungated Annexin-V⁺ 7AAD⁺) is depicted from the same cultures as in B and C. Experiments were repeated twice.