Fig. 5.

Single optical confocal microscopy images showing two types of twin BrdU(+) cells in 7-day labeled brain. Triple immunostaining was done for BrdU (A, F), rb-NG2 (B, G), Olig2 (C, H), and Hoechst 33258 (D, I). (E) and (J) are merged images. Most BrdU(+) twin cells were BrdU(+)/NG2(+)/Olig2(+) (arrows, A–E). While in small number of BrdU(+) twin cells, one was NG2(−) (arrowheads, F–J), but both cells expressed oligodendrocyte lineage marker, Olig2 (F–J). These data suggest that BrdU(+)/NG2(−) cells are differentiating into mature oligodendrocytes. Bar=20 µm.