Figure 2.

The absence of syk−/− B cells in the blood and spleen is not corrected by expression of Ig^HEL. (A) Numbers of B220-positive cells in the spleen. Histograms show arithmetic means and dots represent individual mice which, with the exception of the rag−/− mouse, were all bone marrow chimeras. (B) Numbers and phenotype of B cells in the bone marrow and spleens of Ig^HEL-transgenic syk−/− and syk+/− chimeric mice, measured by two-color flow cytometry of cells stained for CD21 and HEL binding. Windows in plots show Ig^HEL transgenic B cells; the frequency of cells is shown as the percentage of the lymphocyte gate. Plots are each representative of six chimeras.