Fig. 5.
Overexpressing mir-218 attenuates the induction of MAFG expression by cigarette smoke condensate in NHBE. CSC induces MAFG expression 1.54-fold (Scrambled + DMSO vs. Scrambled + CSC). However, when mir-218 is overexpressed, CSC induces MAFG expression only 1.11-fold (mir-218 + CSC vs. mir-218 + DMSO). The significantly reduced induction of MAFG by cigarette smoke condensate in the presence of high levels of mir-218 (2-way ANOVA interaction effect, P = 0.000003) suggests that modulation of mir-218 contributes to the CSC-dependent regulation of MAFG expression.