Fig. 3.

The P element insertion in EY05103 inactivates the gene CG6718 encoding the calcium-independent phospholipase A2, iPLA2VIA. (A) The Drosophila genomic region containing the gene (top bar, adapted from Flybase). The Flybase annotation indicates that CG6718, through alternative splicing, produces 4 mRNA transcripts (RA, RB, RC, and RD) that encode 2 protein products with different N termini (PA and that of PB, PC, or PD). The position of the EY05103 P element insertion is indicated by an open triangle above the genomic region. Two arrowheads below the genomic region and above the mRNAs schematics indicate the positions of the primers used for RT-PCR. (B) Agarose gel electrophoresis of the products generated by RT-PCR from the CG6718-mRNAs in wild type (WT) and the EY05103 (EY) fly strain. Lane EY, homozygous EY05103 insertion strain; lane WT, w¹¹¹⁸ strain; lane M, DNA size marker. RA, RB, RC, and RD correspond to the CG6718 transcripts annotated in the Flybase. The asterisk indicates a new alternatively spliced transcript not yet annotated in the Flybase. Control indicates the amplified transcript fragment of an irrelevant gene (CG34133) as an internal control. The identity of each fragment was confirmed by DNA sequencing.