Figure 2.
Ability of transgenic IFN-γ to induce in the thyroid cells the expression of MHC class I, MHC class II, and CIITA genes. Northern blot analyses were performed by using 20 μg of total RNA extracted from thyroids of normal C57BL6 mice (N) and the three transgenic lines (A, B, and C). Blots were sequentially hybridized with probes for MHC class I, MHC class II, CIITA, and β-actin. The ratio of binding of each probe to β-actin was calculated to correct for the amount of loading.