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Influence of transgenic IFN-γ on the expression of thyroid-specific/restricted genes. Northern analyses were performed by using 20 μg of total RNA extracted from thyroids of normal C57BL6 mice (N) and the three transgenic lines (A, B, and C). Blots were sequentially hybridized with probes for TG, TPO, TSH-R, NIS, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The ratio of binding of each probe to β-actin was calculated to correct for the amount of loading.
