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Ability of transgenic IFN-γ to modulate NIS expression. Western blot analysis was performed by using 100 μg of total proteins extracted from the thyroids of normal C57BL6 mice (N) and the three transgenic lines (A, B, and C). Blots were sequentially hybridized with antibodies for NIS and actin. The ratio of binding of each probe to actin was calculated to correct for loading.
