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. 2000 Feb 4;97(4):1719–1724. doi: 10.1073/pnas.020522597

Figure 6.

Figure 6

The effect of IFN-γ on NIS expression is dose and time dependent. FRTL-5 cells in six hormones were washed with serum-free medium and exposed to different doses of IFN-γ for 2 or 6 days. In the 6-day experiments, the culture medium with IFN-γ was replaced every other day. At the end of the experiments, Northern blot analyses were performed by using 20 μg total of RNA. (A) Blots were sequentially hybridized with probes for NIS, MHC class II (as control), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (to correct for loading). (B) The ratio of binding of each probe to GAPDH was calculated, and this value was set at 1 for MHC class II. NIS values were compared with the corresponding MHC class II control and expressed as relative mRNA levels. Data are the mean ± SD of three different experiments, each performed in duplicate. Single or double asterisks represent a significant difference with P < 0.05 or < 0.01, respectively, by two-way ANOVA.