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. Author manuscript; available in PMC: 2009 Mar 3.
Published in final edited form as: Methods Mol Biol. 2008;474:61–77. doi: 10.1007/978-1-59745-480-3_5

Fig. 2.

Fig. 2

(A) Reversed-phase high-performance liquid chromatographic (RP-HPLC) trace of the crude material isolated from the resin cleavage reaction, Absorbance at 220 nm is monitored versus retention time for peptide A (VKVKVDPPTKVKVKVKVKVKV-NH2) on a C18 column employing a linear gradient of 0% to 100% solvent B in 100min. Desired peptide elutes at 30min. (B) ESI-MS (electrospray ionization mass spectrometry) of crude material isolated from resin cleavage reaction. The molecular ions of the peptide are labeled and defined (inset). Unlabeled peaks in the spectrum are indicative of the impurities in the crude material. (C) Representative sernipreparative RP-HPLC chromatogram. The desired peptide fraction is collected from 45 to 49 min.