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. Author manuscript; available in PMC: 2009 Mar 3.
Published in final edited form as: Methods Mol Biol. 2008;474:61–77. doi: 10.1007/978-1-59745-480-3_5

Table 1.

Semipreparative High-Performance Liquid Chromatographic (HPLC) Gradient Calculated for Peptide A

Time (min) Solvent B (%) Gradient
0 0 —
22 22 1% solvent B per min
312 100 0.25% solvent B per min