Table 1. Study Groups.

Group	Electric Field [V/cm]	# Pulses	Frequency [Hz]	# Animals
1	3500	10	10	5
2	1750	10	10	4
3	875	10	10	4
4	437.5	10	10	4
5	1750	45	4	4
6	875	45	4	4
7	1750	90	4	4
8	875	90	4	4

The table shows the eight different electroporation parameters used in this study. Groups differ in the magnitude of the applied electric field, the number of the pulses and their frequency. All pulses were square pulses, 100 µs in length. A frequency of 10 Hz was used for the 10-pulse protocols, and was reduced to 4 Hz for 45 or 90 pulse protocols to prevent significant heating.